Name : Human HLA-A*02:01&B2M&MAGE-A10 (GLYDGMEHL) Tetramer Protein
Product Source :
Recombinant Human HLA-A*02:01&B2M&MAGE-A10 (GLYDGMEHL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and GLYDGMEHL peptide.[Accession | A0A140T913(HLA-A*02:01)&P61769(B2M)&GLYDGMEHL]
Molecular Weight :
The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human HLA-A*02:01&B2M&MAGE-A10 (GLYDGMEHL) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%. SEC-HPLC The purity of Human HLA-A*02:01&B2M&MAGE-A10 (GLYDGMEHL) Tetramer is greater than 95% as determined by SEC-HPLC.
Background :
MAGE-A10 is a subtype of the Melanoma-associated antigen A (MAGE-A), a class of tumor antigens that are extensively expressed in various histological types of tumors and represents an attractive target for tumor immunotherapy. High-level expression of MAGE-A10 improved the anti-tumor immune cytotoxicity of MAGE-A10-specific CTLs in lung cancer cell lines and primary lung cancer cells.
Synonyms :
MAGE-A10
References & Citations :
Li Z, Guo P, Guo P, Dong K, Liu F, Wu Y, Li J, Shan B, Sang M. 5-aza-2′-deoxycytidine (DAC) treatment induces the MAGE-A10 expression and improves the cytotoxicity of MAGE-A10-specific CTLs in lung cancer cells. Transl Cancer Res. 2020 Feb;9(2):1235-1245. doi: 10.21037/tcr.2020.01.10. PMID: 35117468; PMCID: PMC8798493.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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