Name : Human Peptide Ready HLA-B*07:02&B2M Monomer Protein
Product Source :
Recombinant Human Peptide Ready HLA-B*07:02&B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Val309 (HLA-B*07:02) and Ile21-Met119 (B2M).[Accession | P01889(HLA-B*07:02)&P61769(B2M)]
Molecular Weight :
The protein has a predicted MW of 48.50 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 90% as determined by HPLC
Formulation :
Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Peptide Ready HLA-B*07:02&B2M Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Peptide Ready HLA-B*07:02&B2M Monomer is greater than 90% as determined by SEC-HPLC. ELISA Data Immobilized Human Peptide Ready HLA-B*07:02&B2M Monomer, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA.
Background :
Peptide Ready HLA-B*07:02&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-B*07:02. Peptide ready MHC molecules comprising human HLA-B*07:02 alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
Synonyms :
MHC; HLA-B; Peptide Ready
References & Citations :
[1] Moritz A, Anjanappa R, Wagner C, Bunk S, Hofmann M, Pszolla G, Saikia A, Garcia-Alai M, Meijers R, Rammensee HG, Springer S, Maurer D. High-throughput peptide-MHC complex generation and kinetic screenings of TCRs with peptide-receptive HLA-A*02:01 molecules. Sci Immunol. 2019 Jul 19;4(37):eaav0860.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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