Name : Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein
Product Source :
Recombinant Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains His24-Pro297 (H-2K(b)), Ile21-Met119(B2M) and SIINFEKL peptide.[Accession | P01901(H-2K(b))&P01887(B2M)&SIINFEKL]
Molecular Weight :
The protein has a predicted MW of 50.20 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse H-2K (b) &B2M&OVA (SIINFEKL) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse H-2K (b) &B2M&OVA (SIINFEKL) Monomer is greater than 95% as determined by SEC-HPLC.
Background :
Ovalbumin (OVA) has been historically a popular source of such antigens, since OVA can induce both humoral and cellular immune responses based on well-characterised peptide epitopes. The OVA257-264 octapeptide was one of the frst OVA epitopes to be characterised, it has an amino acid sequence SIINFEKL, which is recognised by cytotoxic T lymphocytes. SIINFEKL forms fbrillar assemblies similar to other peptide hydrogels. Te immunoactive properties of this peptide can therefore be related to its self-assembling nature.
Synonyms :
H-2K; OVA; MHC; H2-K1;
References & Citations :
Kamalov M, Kählig H, Rentenberger C, Müllner ARM, Peterlik H, Becker CFW. Ovalbumin Epitope SIINFEKL Self-Assembles into a Supramolecular Hydrogel. Sci Rep. 2019 Feb 25;9(1):2696. doi: 10.1038/s41598-019-39148-8. PMID: 30804439; PMCID: PMC6390181.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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