Name : Biotinylated Human Midkine Protein
Product Source :
Recombinant Biotinylated Human Midkine Protein is expressed from E.coli with His tag and Avi Tag at the N-Terminus. It contains Val21-Asp143.[Accession | P21741-1]
Molecular Weight :
The protein has a predicted MW of 16.46 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human Midkine on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Background :
Midkine is a heparin-binding growth factor, originally reported as the product of a retinoic acid-responsive gene during embryogenesis, but currently viewed as a multifaceted factor contributing to both normal tissue homeostasis and disease development. Midkine is abnormally expressed at high levels in various human malignancies and acts as a mediator for the acquisition of critical hallmarks of cancer, including cell growth, survival, metastasis, migration, and angiogenesis.
Synonyms :
MK; ARAP; MDK; MEK; MK1; MKARAP; NEGF2
References & Citations :
Filippou PS, Karagiannis GS, Constantinidou A. Midkine (MDK) growth factor: a key player in cancer progression and a promising therapeutic target. Oncogene. 2020 Mar;39(10):2040-2054. doi: 10.1038/s41388-019-1124-8. Epub 2019 Dec 4. PMID: 31801970.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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