Name : Human MICA Protein
Product Source :
Recombinant Human MICA Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu24-Gln308.[Accession | Q96QC4]
Molecular Weight :
The protein has a predicted MW of 35.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human MICA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human MICA is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human MICA, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.88μg/ml determined by ELISA (QC Test). ELISA Data Immobilized Human MICA, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-MICA Antibody, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA.
Background :
MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig‑like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin..
Synonyms :
FLJ60820; MGC111087; MICA; PERB11.1; DAMA-345G11.2; FLJ36918; FLJ60820; MGC21250
References & Citations :
(1)Friese M A , Platten M , Lutz S Z , et al. MICA/NKG2D-mediated immunogene therapy of experimental gliomas.[J]. Cancer Research, 2003, 63(24):8996-9006.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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