Name : Human MIF Protein
Product Source :
Recombinant Human MIF Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Pro2-Ala115.[Accession | P14174]
Molecular Weight :
The protein has a predicted MW of 13.44 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human MIF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Background :
Macrophage migration inhibitory factor (MIF) is a pleiotropic cytokine with chemokine-like functions that increasingly is being studied in different aspects of cardiovascular disease. MIF was first identified as a proinflammatory and pro-survival mediator within the immune system, and a second structurally related MIF family member, D-dopachrome tautomerase (a.k.a. MIF-2), was reported recently.
Synonyms :
GIF; L-dopachrome isomerase; GLIF; MMIF; EC 5.3.2.1; EC 5.3.3.12
References & Citations :
Tilstam PV, Qi D, Leng L, Young L, Bucala R. MIF family cytokines in cardiovascular diseases and prospects for precision-based therapeutics. Expert Opin Ther Targets. 2017 Jul;21(7):671-683. doi: 10.1080/14728222.2017.1336227. PMID: 28562118; PMCID: PMC6130320.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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