Name : Biotinylated Human MMP-9 Protein
Product Source :
Recombinant Biotinylated Human MMP-9 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala20-Asp707.[Accession | P14780]
Molecular Weight :
The protein has a predicted MW of 79.3 kDa. Due to glycosylation, the protein migrates to 95-105 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human MMP-9 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human MMP-9 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Anti-MMP-9 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human MMP-9, His Tag with the EC50 of 14.3ng/ml determined by ELISA.
Background :
Matrix metalloproteinase 9 (MMP9) contributes to this process and deficiencies in the MMP9 lead to impaired healing. Inappropriate expression of MMP9 also contributes to impaired re-epithelialization. Previously we demonstrated that FOXO1 was activated in wound healing but to higher levels in diabetic wounds. To address mechanisms of impaired re-epithelialization we examined MMP9 expression in vivo in full thickness dermal scalp wounds created in experimental K14.
Synonyms :
MMP-9; GELB; CLG4B; MANDP2; Gelatinase B
References & Citations :
(1)Zhang C, Lim J, Jeon HH, et al. FOXO1 deletion in keratinocytes improves diabetic wound healing through MMP9 regulation. Sci Rep. 2017;7(1):10565. Published 2017 Sep 5. doi:10.1038/s41598-017-10999-3
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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