Name : Mouse MMP-8 Protein (pro form)

Product Source :
Recombinant Mouse MMP-8 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Phe21-Ser465.[Accession | O70138]

Molecular Weight :
The protein has a predicted MW of 51.9 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Supplied as 0.22μm filtered solution in 50mM Tris, 10mM CaCl2, 150mM NaCl (pH 7.5).

Reconstitution :

Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Mouse MMP-8 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse MMP-8 is greater than 95% as determined by SEC-HPLC.

Background :
Alteration of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) expression has been studied for various cardiac diseases, including dilated cardiomyopathy (DCM), with the significance of surrogate markers of extracellular matrix (ECM) remodeling. MMP-8 was identified only in myocardiocytes, while MMP-9 and TIMP-2 were present in both myocardiocytes and stroma, but with different intensity. The increasing intensity of MMP-8 and TIMP-2 immunoreactions was significantly associated with low HCS.

Synonyms :
MMP-8; PMNL-CL; CLG1; MMP8; Collagenase 2; PMNL-CL; HNC

References & Citations :
(1) Mitruţ R, Stepan AE, Mărgăritescu C, Andreiana BC, Kesse AM, Simionescu CE, Militaru C. Immunoexpression of MMP-8, MMP-9 and TIMP-2 in dilated cardiomyopathy. Rom J Morphol Embryol. 2019;60(1):119-124. PMID: 31263835.

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