Name : Human MARCO Protein
Product Source :
Recombinant Human MARCO Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Ser405-Val520.[Accession | Q9UEW3-1]
Molecular Weight :
The protein has a predicted MW of 13.8 kDa. Due to glycosylation, the protein migrates to 14-20 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human MARCO on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human MARCO is greater than 95% as determined by SEC-HPLC.
Background :
Macrophage receptor with collagenous structure (MARCO) is the predominant scavenger receptor for recognition and binding of silica particles by alveolar macrophages (AM). MARCO contributes to normal cholesterol uptake in macrophages; therefore, in the absence of MARCO, macrophages are more susceptible to a greater inflammatory response by particulates known to cause NLRP3 inflammasome activation and the effect is due to increased LMP.
Synonyms :
MARCO; SCARA2; AI323439; Ly112
References & Citations :
Biswas R, Hamilton RF Jr, Holian A. Role of lysosomes in silica-induced inflammasome activation and inflammation in absence of MARCO. J Immunol Res. 2014;2014:304180. doi: 10.1155/2014/304180. Epub 2014 Jun 26. PMID: 25054161; PMCID: PMC4099041.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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