Name : Human NKp30/NCR3/CD337 Protein
Product Source :
Recombinant Human NKp30/NCR3/CD337 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Leu19-Thr138.[Accession | AAH52582]
Molecular Weight :
The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per ug by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human NKp30 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human NKp30 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human NKp30, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-NKp30 Antibody, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test). SPR Data Human NKp30, hFc Tag captured on CM5 Chip via Protein A can bind Human B7-H6, His Tag with an affinity constant of 23.10 nM as determined in SPR assay (Biacore T200).
Background :
NKp30, along with NKp44 and NKp46, constitute a group of receptors termed “Natural Cytotoxicity Receptors”. These receptors play a major role in triggering NK-mediated killing of most tumor cells lines.NKp30 stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It controls, for instance, NK cells cytotoxicity against tumor cells. Engagement of NCR3 by BAG6 also promotes myeloid dendritic cells (DC) maturation, both through killing DCs that did not acquire a mature phenotype, and inducing the release by NK cells of TNFA and IFNG which promote DC maturation.
Synonyms :
CD337; LY117; NCR3; NKp30; 1C7; MALS
References & Citations :
(1) Strandmann E P V , Shatnyeva O , Hansen H P. NKp30 and its ligands: Emerging players in tumor immune evasion from natural killer cells[J]. Annals of Translational Medicine, 2015.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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