Name : Human Notch 1 Protein

Product Source :
Recombinant Human Notch 1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala19-Gln526.[Accession | P46531]

Molecular Weight :
The protein has a predicted MW of 56.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human Notch 1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. SEC-HPLC The purity of Human Notch 1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human Notch 1, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human DLL4, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA (QC Test). SPR Data Human DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Human Notch 1, His Tag with an affinity constant of 0.48 μM as determined in SPR assay (Biacore T200).

Background :
Human Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes.Notch-1 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

Synonyms :
Notch-1; TAN1; NOTCH1; 9930111A19Rik; lin-12; Mis6; N1

References & Citations :
(1)Hales E C , Taub J W , Matherly L H. New insights into Notch1 regulation of the PI3K–AKT–mTOR1 signaling axis: Targeted therapy of γ-secretase inhibitor resistant T-cell acute lymphoblastic leukemia[J]. Cellular Signalling, 2014, 26(1):149-161.

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