Name : Human Notch 2 Protein
Product Source :
Recombinant Human Notch 2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Leu26-GIn530.[Accession | Q04721]
Molecular Weight :
The protein has a predicted MW of 57 kDa. Due to glycosylation, the protein migrates to 68-78 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Notch 2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Notch 2 is greater than 95% as determined by SEC-HPLC.
Background :
Human Notch 2 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes. Although Notch proteins are structurally and functionally similar, deletion of either Notch-1 or Notch 2 is lethal, showing that not all functions overlap.Notch 2 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus.
Synonyms :
AGS2; Notch-2; NOTCH2; N2ECD; HJCYS; N2
References & Citations :
(1)Wu Y , Cain-Hom C , Choy L , et al. Therapeutic antibody targeting of individual Notch receptors[J]. NATURE, 2010, 464(7291):1052-1057.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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