Name : SARS-COV-2 NSP7&NSP8 Protein
Product Source :
SARS-COV-2 NSP7&NSP8 Protein is expressed from E.coil with His tag at the C-Terminus. It contains Ser1-Gln83(NSP7)&Ala1-Gln198(NSP8).[Accession | YP_009725303.1(NSP7)&YP_009725304.1(NSP8)]
Molecular Weight :
The protein has a predicted MW of 32.8 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM Nacl, 200mM Arginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE SARS-COV-2 NSP7&NSP8 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Background :
The crystal structure of the metabolite of remdesivir (Monophosphate of GS-441524) and NSP12-NSP8-NSP7 of SARS CoV-2 virus was recently reported. The crystal structures of ADP-Ribose or AMP and NSP3 of SARS CoV-2 virus were also released, recently. The crystal structure of NSP3 of SARS CoV-2 virus as an alternative binding site of AMP or ADP-ribose to treat COVID-19.
Synonyms :
SP7&NSP8; nsp7&nsp8
References & Citations :
(1) Jung LS, Gund TM, Narayan M. Comparison of Binding Site of Remdesivir and Its Metabolites with NSP12-NSP7-NSP8, and NSP3 of SARS CoV-2 Virus and Alternative Potential Drugs for COVID-19 Treatment. Protein J. 2020 Dec;39(6):619-630. doi: 10.1007/s10930-020-09942-9. Epub 2020 Nov 13. PMID: 33185784; PMCID: PMC7662030.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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