Name : Human OGN/Osteoglycin Protein
Product Source :
Recombinant Human OGN/Osteoglycin Protein is expressed from HEK293 with His tag at the C-terminus. It contains Pro21-Phe298.[Accession | P20774]
Molecular Weight :
The protein has a predicted MW of 32.83 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human OGN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Background :
Osteoglycin (OGN, a.k.a. mimecan) belongs to cluster III of the small leucine-rich proteoglycans (SLRP) of the extracellular matrix (ECM). In vertebrates OGN is a characteristic ECM protein of bone. The function of OGN has mainly been studied in mammals in which it regulates collagen fibrillogenesis, the efficiency of which is increased when it is processed by BMP-1/Tolloid-like metalloproteinases. OGN has a role in wound healing in the cornea, in ath_x005f_x0002_erosclerotic lesions and modulates myocardial integrity and remodelling. In addition, OGN enhances the neurite outgrowth promoted by insulin-like growth factor-2 and IGF binding protein-2.
Synonyms :
Osteoglycin; OIF; OGN; SLRR3A
References & Citations :
Costa RA, Martins RST, Capilla E, Anjos L, Power DM. Vertebrate SLRP family evolution and the subfunctionalization of osteoglycin gene duplicates in teleost fish. BMC Evol Biol. 2018 Dec 13;18(1):191. doi: 10.1186/s12862-018-1310-2. PMID: 30545285; PMCID: PMC6293640.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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