Name : Human OSMR&GP130 Protein
Product Source :
Recombinant Human OSMR&GP130 Protein is expressed from HEK293 with His tag and Flag tag at the C-Terminus. It contains Glu28-Met740(OSMR)&Glu23-Glu619(GP130).[Accession | Q99650-1(OSMR)&P40189-1(GP130)]
Molecular Weight :
The protein has a predicted MW of 82.2 kDa(OSMR) and 68.83 kDa(GP130). Due to glycosylation, the protein migrates to 100-140 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human OSMR&GP130 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human OSMR&GP130 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human OSMR&GP130, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA.
Background :
OSMR&gp130 is a type II receptor for OSM in humans. OSM can alternatively bind to a heterodimer of gp130 and LIF-R or of gp130 and OSMR (OSM receptor). On the basis of in vitro studies, the OSM ligand recruits a broader array of cell signaling pathways than most gp130 cytokines. OSMR and gp130 chains are widely expressed in connective tissue cells and tumor cells.
Synonyms :
References & Citations :
(1) Richards CD. The enigmatic cytokine oncostatin m and roles in disease. ISRN Inflamm. 2013 Dec 8;2013:512103. doi: 10.1155/2013/512103. PMID: 24381786; PMCID: PMC3870656.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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