Name : Mouse NTS1 Protein

Product Source :
Recombinant Mouse NTS1 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ser23-Leu162.[Accession | Q9D3P9]

Molecular Weight :
The protein has a predicted MW of 42.9 kDa. Due to glycosylation, the protein migrates to 48-51 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE

Formulation :
Lyophilized from 0.22μm filtered solution inPBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Mouse NTS1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Background :
Crystal structures of neurotensin receptor subtype 1 (NTS1) allowed us to visualize the binding mode of the endogenous peptide hormone neurotensin and its pharmacologically active C-terminal fragment NT(8-13) within the orthosteric binding pocket of NTS1.

Synonyms :
NT; NTR; NTR1; NTRH; NTRR; NTS1; NTSR1; NMN-125; NN; NT/N

References & Citations :
(1) Kling RC, Burchardt C, Einsiedel J, Hübner H, Gmeiner P. Structure-based exploration of an allosteric binding pocket in the NTS1 receptor using bitopic NT(8-13) derivatives and molecular dynamics simulations. J Mol Model. 2019 Jun 17;25(7):193. doi: 10.1007/s00894-019-4064-x. PMID: 31209646.

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