Name : Biotinylated Human PD-1/PDCD1 Protein
Product Source :
Recombinant Biotinylated Human PD-1/PDCD1 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus. It contains Leu25-Gln167.[Accession | Q15116-1]
Molecular Weight :
The protein has a predicted MW of 44.7 kDa. Due to glycosylation, the protein migrates to 65-72 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human PD-1 on Tris-Bis PAGE under reduced. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human PD-1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human PD-L1, mFc tag at 2μg/ml (100μl/Well). Dose response curve for Biotinylated Human PD-1, hFc tag with the EC50 of 0.23μg/ml determined by ELISA.
Background :
Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system’s response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.
Synonyms :
PDCD1; PD1; CD279; SLEB2; PD-1
References & Citations :
(1)Blank C , Mackensen A. Contribution of the PD-L1/PD-1 pathway to T-cell exhaustion: an update on implications for chronic infections and tumor evasion[J]. Cancer Immunology Immunotherapy, 2007, 56(5):739-745.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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