Name : Canine PDGFA Protein
Product Source :
Recombinant Canine PDGFA Protein is expressed from Yeast with His tag at the N-terminus. It contains Ser87-Arg196.[Accession | A0A8C0M6T8]
Molecular Weight :
The protein has a predicted MW of 13.62 kDa. Due to glycosylation, the protein migrates to 15-20 kDa and 35-45 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 90% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in 4mM HCL. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 4mM HCL.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Canine PDGFA on Tris-Bis PAGE under reduced condition. The purity is greater than 90%. Cell Based Assay Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is 5 – 15 ng/mL.
Background :
Platelet-derived growth factors (PDGFs) may play an important role in the development of atherosclerosis acting as chemoattractants and mitogens for vascular smooth muscle cells and macrophages. PDGFA, the most known member of PDGF family, plays a crucial role in occurrence and progression of different tumors.
Synonyms :
References & Citations :
(1) Herrmann SM, Ricard S, Nicaud V, Brand E, Behague I, Blanc H, Ruidavets JB, Evans A, Arveiler D, Luc G, Poirier O, Cambien F. Polymorphisms in the genes encoding platelet-derived growth factor A and alpha receptor. J Mol Med (Berl). 2000;78(5):287-92. doi: 10.1007/s001090000111. PMID: 10954201.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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