Name : Human PLA2G1B Protein
Product Source :
Recombinant Human PLA2G1B Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala23-Ser148.[Accession | P04054]
Molecular Weight :
The protein has a predicted MW of 15.23 kDa. Due to glycosylation, the protein migrates to 18-23 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in 10mM Tris, 5mM CaCl2 (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human PLA2G1B on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human PLA2G1B is greater than 95% as determined by SEC-HPLC.
Background :
Phospholipase A2 (PLA2) plays crucial roles in diverse cellular responses, including phospholipid digestion and metabolism, host defense and signal transduction. PLA2 provides precursors for generation of eicosanoids, such as prostaglandins (PGs) and leukotrienes (LTs), when the cleaved fatty acid is arachidonic acid, platelet-activating factor (PAF) when the sn-1 position of the phosphatidylcholine contains an alkyl ether linkage and some bioactive lysophospholipids, such as lysophosphatidic acid (lysoPA).
Synonyms :
Phospholipase A2; PLA2G1B; PLA2; PLA2A; PLA2AMGC119834
References & Citations :
(1) Murakami M, Nakatani Y, Atsumi GI, Inoue K, Kudo I. Regulatory Functions of Phospholipase A2. Crit Rev Immunol. 2017;37(2-6):127-195. doi: 10.1615/CritRevImmunol.v37.i2-6.20. PMID: 29773019.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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