Name : Mouse PLA2G7 Protein
Product Source :
Recombinant Mouse PLA2G7 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Phe22-Asn440.[Accession | Q60963]
Molecular Weight :
The protein has a predicted MW of 47.9 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse PLA2G7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse PLA2G7 is greater than 95% as determined by SEC-HPLC.
Background :
Endogenous PLA2G7 transcription levels were found to be significantly lower in vascular related cell lines than in the other cell lines. Luciferase reporter gene assays indicated that gene activity was significantly enhanced by PLA2G7 promoter fragment. LA2G7 transcription was found to be up-regulated with the treatment of DAC. The 17-β-estradiol was found to down-regulate PLA2G7 transcription in all the cell lines.
Synonyms :
LDL-PLA2; PAFAD; PAFAH
References & Citations :
(1) Jiang D, Wang Y, Shen Y, Xu Y, Zhu H, Wang J, Wang H, Duan S. Estrogen and promoter methylation in the regulation of PLA2G7 transcription. Gene. 2016 Oct 10;591(1):262-267. doi: 10.1016/j.gene.2016.07.048. Epub 2016 Jul 21. PMID: 27450918.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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