Name : Biotinylated Mouse PLAU/uPA Protein
Product Source :
Recombinant Biotinylated Mouse PLAU/uPA Protein is expressed from HEK293 with His tag and Avi Tag at the C-Terminus. It contains Gly21-Phe433.[Accession | P06869]
Molecular Weight :
The protein has a predicted MW of 49.00 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Mouse PLAU on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Mouse PLAU is greater than 95% as determined by SEC-HPLC. SPR Data Mouse uPAR isoform 1, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse PLAU, His-Avi Tag with an affinity constant of 0.19 μM as determined in SPR assay (Biacore T200).
Background :
Plasminogen activator, urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers. PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC.
Synonyms :
PLAU; Urokinase; ATF; UPA; URK; u-PA; BDPLT5; QPD
References & Citations :
(1) Chen G, Sun J, Xie M, Yu S, Tang Q, Chen L. PLAU Promotes Cell Proliferation and Epithelial-Mesenchymal Transition in Head and Neck Squamous Cell Carcinoma. Front Genet. 2021 May 20;12:651882. doi: 10.3389/fgene.2021.651882. PMID: 34093649; PMCID: PMC8173099.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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