Name : Mouse PODXL2 Protein
Product Source :
Recombinant Mouse PODXL2 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Val29-Thr499.[Accession | Q8CAE9]
Molecular Weight :
The protein has a predicted MW of 51.7 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse PODXL2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse PODXL2 is greater than 95% as determined by SEC-HPLC.
Background :
Transmembrane protein endoglycan (PODXL2), which belongs to the CD34-family of highly glycosylated sialomucins. Endoglycan is broadly expressed in the developing mouse brains and is proteolytically shed in vitro in mouse neurons and in vivo in mouse brains. Endoglycan shedding in primary neurons was mediated by the transmembrane protease a disintegrin and metalloprotease 10 (ADAM10), but not by its homolog ADAM17.
Synonyms :
Endoglycan; PODLX2; brain-1; BRN1; EG; oct-8; OTF8; UNQ1861/PRO3742
References & Citations :
(1) Hsia HE, Tüshaus J, Feng X, Hofmann LI, Wefers B, Marciano DK, Wurst W, Lichtenthaler SF. Endoglycan (PODXL2) is proteolytically processed by ADAM10 (a disintegrin and metalloprotease 10) and controls neurite branching in primary neurons. FASEB J. 2021 Sep;35(9):e21813. doi: 10.1096/fj.202100475R. PMID: 34390512.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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