Name : Human PSMA/FOLH1 Protein
Product Source :
Recombinant Human PSMA/FOLH1 Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Lys44-Ala750.[Accession | Q04609-1]
Molecular Weight :
The protein has a predicted MW of 106.8 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human PSMA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human PSMA is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human PSMA, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-PSMA Antibody, hFc Tag with the EC50 of 16.3ng/ml determined by ELISA.
Background :
Prostate-specific membrane antigen (PSMA) is an enzyme that in humans is encoded by the FOLH1 (folate hydrolase 1) gene, also known as Glutamate carboxypeptidase II (GCPII). Human PSMA is highly expressed in the prostate, roughly a hundred times greater than in most other tissues. In some prostate cancers, PSMA is the second-most upregulated gene product, with an 8- to 12-fold increase over levels in noncancerous prostate cells.
Synonyms :
GCP2; PSMA; PSMAFGCP; FGCP; Folate hydrolase 1; FOLHNAALADase I; GCPII; GCPIImGCP; NAALAD1; NAALADase I; NAALAdase; FOLH1; GIG27; PSM; FOLH; mGCP
References & Citations :
(1)Song C , Francesca Z , Williams D G , et al. Antitumor Activity of MEDI3726 (ADCT-401), a Pyrrolobenzodiazepine Antibody-drug Conjugate Targeting PSMA, in Pre-clinical Models of Prostate Cancer[J]. Molecular Cancer Therapeutics, 2018:molcanther.0982.2017-.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
SUSD4 Protein
Latexin Protein
Popular categories:
A Disintegrin and Metalloprotease 22
Cathepsin L1