Name : Human PSMP Protein
Product Source :
Recombinant Human PSMP Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Lys37-Ser139.[Accession | Q1L6U9]
Molecular Weight :
The protein has a predicted MW of 37.9 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human PSMP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human PSMP is greater than 95% as determined by SEC-HPLC.
Background :
PC3-secreted microprotein (PSMP)/microseminoprotein (MSMP) is a novel chemotactic cytokine and its receptor is CCR2. PSMP was highly expressed in fibrotic/cirrhotic tissues from patients with different etiologies of liver disease and in the 3 experimental mouse models of fibrosis. Damage-associated molecular pattern molecules HMGB-1 and IL-33 induced hepatocytes to produce PSMP. PSMP promotes liver fibrosis through inflammatory macrophage infiltration, polarization and production of proinflammatory cytokines, as well as direct activation of hepatic stellate cells via its receptor CCR2.
Synonyms :
Msmp; Psmp; PC3-secreted microprotein
References & Citations :
(1) She S, Wu X, Zheng D, Pei X, Ma J, Sun Y, Zhou J, Nong L, Guo C, Lv P, Song Q, Zheng C, Liang W, Huang S, Li Q, Liu Z, Song Z, Li Y, Zhang Y, Kong W, You H, Xi J, Wang Y. PSMP/MSMP promotes hepatic fibrosis through CCR2 and represents a novel therapeutic target. J Hepatol. 2020 Mar;72(3):506-518. doi: 10.1016/j.jhep.2019.09.033. Epub 2019 Dec 6. PMID: 31813573.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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