Name : Human CD45/PTPRC Protein
Product Source :
Recombinant Human CD45/PTPRC Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gln26-Lys577.[Accession | P08575-3]
Molecular Weight :
The protein has a predicted MW of 87.53 kDa. Due to glycosylation, the protein migrates to 120-160 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CD45 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CD45 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized AHuman CD45, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-CD45 Antibody, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA.
Background :
PTPRC (also known as CD45),T cells require the protein tyrosine phosphatase CD45 to detect and respond to antigen because it activates the Src family kinase Lck, which phosphorylates the T cell antigen receptor (TCR) complex. CD45 ativates Lck by opposing the negative regulatory kinase Csk. Paradoxically, CD45 has also been implicated in suppressing TCR signaling by dephosphorylating the same signaling motifs within the TCR complex upon which Lck acts.
Synonyms :
L-CA; CD45; T200; PTPRC; B220; CD45R; GP180; LCA; LY5; EC 3.1.3.48
References & Citations :
(1) Courtney AH, Shvets AA, Lu W, Griffante G, Mollenauer M, Horkova V, Lo WL, Yu S, Stepanek O, Chakraborty AK, Weiss A. CD45 functions as a signaling gatekeeper in T cells. Sci Signal. 2019 Oct 22;12(604):eaaw8151. doi: 10.1126/scisignal.aaw8151. PMID: 31641081; PMCID: PMC6948007.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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