Name : Cynomolgus B7-H2/ICOSLG Protein
Product Source :
Recombinant Cynomolgus B7-H2/ICOSLG Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Asp19-Thr256.[Accession | XP_005548617.1]
Molecular Weight :
The protein has a predicted MW of 27.7 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus B7-H2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus B7-H2 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human ICOS, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus B7-H2, His Tag with the EC50 of 12.6ng/ml determined by ELISA (QC Test). SPR Data Human ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus B7-H2, His Tag with an affinity constant of 5.42 nM as determined in SPR assay (Biacore T200).
Background :
B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.
Synonyms :
CD275; B7H2; B7-H2; B7RP-1; GL50; ICOSL; ICOS-L; ICOSLG; B7RP1; LICOS; ICOS ligand; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50
References & Citations :
(1)Yao S , Zhu Y , Chen L. Advances in targeting cell surface signalling molecules for immune modulation[J]. Nature Reviews Drug Discovery, 2013, 12(2):130-146.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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