Name : Human PVRIG Protein

Product Source :
Recombinant Human PVRIG Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Thr41-Asp171.[Accession | Q6DKI7]

Molecular Weight :
The protein has a predicted MW of 40.42 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human PVRIG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human PVRIG is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human PVRIG, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-PVRIG Antibody, hFc Tag with the EC50 of 10.9ng/ml determined by ELISA.

Background :
Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family.PVRIG is a cell surface receptor for NECTIN2. May act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals. Following interaction with NECTIN2, inhibits T-cell proliferation. Competes with CD226 for NECTIN2-binding.

Synonyms :
C7orf15; CD112R; MGC104322; MGC138297; MGC2463; PVRIG; ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2; RalGDS/AF-6; RMO1

References & Citations :
(1)PVRIG and PVRL2 Are Induced in Cancer and Inhibit CD8 T-cell Function. 2019.

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