Name : Biotinylated Mouse PVRIG Protein
Product Source :
Recombinant Biotinylated Mouse PVRIG Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus. It contains Ser35-Asp165.[Accession | A0A1B0GS01]
Molecular Weight :
The protein has a predicted MW of 42.7 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Mouse PVRIG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Mouse Nectin-2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse PVRIG, hFc Tag with the EC50 of 1.76μg/ml determined by ELISA (QC Test). SPR Data Biotinylated Mouse PVRIG, hFc-Avi Tag captured on CM5 Chip via Protein A can bind Mouse Nectin-2, His Tag with an affinity constant of 2.22 μM as determined in SPR assay (Biacore T200).
Background :
Murine PVRIG interacted weakly with poliovirus receptor (PVR) but bound poliovirus receptor-like 2 (PVRL2) strongly, making the latter its principal ligand.PVRIG is an inducible checkpoint receptor and that targeting PVRIG-PVRL2 interactions results in increased CD8+ T-cell function and reduced tumor growth.
Synonyms :
C7orf15; CD112R; MGC104322; MGC138297; MGC2463; PVRIG; ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2; RalGDS/AF-6; RMO1
References & Citations :
Murter B, Pan X, Ophir E, Alteber Z, Azulay M, Sen R, Levy O, Dassa L, Vaknin I, Fridman-Kfir T, Salomon R, Ravet A, Tam A, Levin D, Vaknin Y, Tatirovsky E, Machlenkin A, Pardoll D, Ganguly S. Mouse PVRIG Has CD8+ T Cell-Specific Coinhibitory Functions and Dampens Antitumor Immunity. Cancer Immunol Res. 2019 Feb;7(2):244-256. doi: 10.1158/2326-6066.CIR-18-0460. Epub 2019 Jan 18. PMID: 30659055; PMCID: PMC7038785.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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