Name : Human RGM-C Protein
Product Source :
Recombinant Human RGM-C Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln33-Asp393.[Accession | Q6ZVN8-1]
Molecular Weight :
The protein has a predicted MW of 40 kDa (mature)&26 kDa (C-terminus peptide)&14 kDa (N-terminus peptide). Due to enzyme lysis and glycosylation, the protein migrates to 55-60 kDa&30-40 kDa&20-25 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human RGM-C on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human RGM-C is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human RGM-C, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA.
Background :
RGM gene family (‘Repulsive Axonal Guidance molecules’ A, B and C),both RGM A and B are mostly expressed in central nervous system, while RGM C is exclusively expressed in all striated muscle and in the myocardium. RGM A and B appear at every level of the developing neural axis, where they colocalize to a large extent in the mantle layer, although only RGM A appears in the neuroepithelium, and only RGM B in the peripheral nervous system.
Synonyms :
Hemojuvelin; Rgmc; DL-M; HFE2; HFE2AMGC23953; HJV; JH; RGMC; RGM-C
References & Citations :
(1) Oldekamp J, Krämer N, Alvarez-Bolado G, Skutella T. Expression pattern of the repulsive guidance molecules RGM A, B and C during mouse development. Gene Expr Patterns. 2004 May;4(3):283-8. doi: 10.1016/j.modgep.2003.11.008. PMID: 15053976.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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