Name : Human RANKL/TNFSF11/CD254 Protein
Product Source :
Recombinant Human RANKL/TNFSF11/CD254 Protein is expressed from HEK293 without tag. It contains Gly63-Asp244.[Accession | O14788-2]
Molecular Weight :
The protein has a predicted MW of 20.5 kDa. Due to glycosylation, the protein migrates to 30-60 kDa based on Tris-Bis PAGE resu
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 90% as determined by Tris-Bis PAGE> 90% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human RANKL on Tris-Bis PAGE under reduced condition. The purity is greater than 90%. SEC-HPLC The purity of Human RANKL is greater than 90% as determined by SEC-HPLC. ELISA Data Immobilized Human RANKL, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 13.6ng/ml determined by ELISA (QC Test). SPR Data Human TNFRSF11B, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human RANKL, No Tag with an affinity constant of 0.030 nM as determined in SPR assay (Biacore T200).
Background :
Receptor activator of nuclear factor x03BA;B (RANK) and its ligand (RANKL) have originally been described for their key roles in bone metabolism and the immune system. Subsequently, it has been shown that the RANKL-RANK system is critical in the formation of mammary epithelia in lactating females and the thermoregulation of the central nervous system. RANKL and RANK are under the tight control of the female sex hormones estradiol and progesterone.
Synonyms :
ODF; OPGL; RANKL; TRANCE; CD254; sOdf; TNFSF11; OPTB2
References & Citations :
(1) Nagy V, Penninger JM. The RANKL-RANK Story. Gerontology. 2015;61(6):534-42. doi: 10.1159/000371845. Epub 2015 Feb 14. PMID: 25720990.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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