Name : Human RNF43 Protein
Product Source :
Recombinant Human RNF43 Protein is expressed from HEK293 with His tag at the C-terminus. It contains Gly24-Tyr197.[Accession | Q68DV7-1]
Molecular Weight :
The protein has a predicted MW of 20.14 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human RNF43 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human RNF43 is greater than 95% as determined by SEC-HPLC. SPR Data Human R-Spondin 3, His Tag immobilized on CM5 Chip can bind Human RNF43, His Tag with an affinity constant of 0.255 μM as determined in SPR assay (Biacore T200).
Background :
RNF43 (E3 ubiquitin-protein ligase RNF43 or RING-type E3 ubiquitin transferase RNF43) functions as a tumor suppressor, by exerting a predominant negative feedback mechanism in the Wnt/β-catenin signaling pathway. RNF43 inhibits Wnt/beta-catenin signaling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation.
Synonyms :
E3 ubiquitin-protein ligase RNF43;RING finger protein 43;RING-type E3 ubiquitin transferase RNF43;RNF43
References & Citations :
(1) Serra S, Chetty R. Rnf43. J Clin Pathol. 2018 Jan;71(1):1-6. doi: 10.1136/jclinpath-2017-204763. Epub 2017 Oct 10. PMID: 29018044.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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