Name : Human MSPR/Ron Protein

Product Source :
Recombinant Human MSPR/Ron Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Glu25-Leu571.[Accession | CAA49634.1]

Molecular Weight :
The protein has a predicted MW of 59.71 kDa. Due to furin cleavage, the protein migrates to 70-80 kDa (full length) and 40-50 kDa (alpha chain) and 35-40 kDa (partial beta chain) based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE

Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human MSPR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Background :
Macrophage-stimulating protein (MSP) is a serum protein belonging to the plasminogen-related growth factor family. The specific receptor for MSP is the RON (recepteur d’origine nantais) receptor tyrosine kinase – a member of the MET proto-oncogene family. Activation of RON by MSP exerts dual functions on macrophages.

Synonyms :
MSP receptor; CDw136; p185-Ron; CD136; MSP R; MSP receptor; MSPR; MST1R; PTK8; RON

References & Citations :
Wang MH, Zhou YQ, Chen YQ. Macrophage-stimulating protein and RON receptor tyrosine kinase: potential regulators of macrophage inflammatory activities. Scand J Immunol. 2002 Dec;56(6):545-53. doi: 10.1046/j.1365-3083.2002.01177.x. PMID: 12472665.

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