Name : Human ROBO4 Protein
Product Source :
Recombinant Human ROBO4 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala27-Glu469.[Accession | Q8WZ75-1]
Molecular Weight :
The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human ROBO4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human ROBO4 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human ROBO4, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-ROBO4 Antibody, hFc Tag with the EC50 of 6.8ng/ml determined by ELISA.
Background :
Roundabout4 (Robo4) is a transmembrane receptor that belongs to the Roundabout (Robo) family of axon guidance molecules. Robo4 is an endothelial-specific receptor that participates in endothelial cell migration, proliferation, and angiogenesis and the maintenance of vasculature homeostasis. Robo4 is a promising and potentially valuable therapeutic target for treating pathological angiogenesis and developmental defects in angiogenesis.
Synonyms :
ECSM4; MRB; FLJ20798; MGC133352; MGC133353; ROBO4
References & Citations :
(1) Dai C, Gong Q, Cheng Y, Su G. Regulatory mechanisms of Robo4 and their effects on angiogenesis. Biosci Rep. 2019 Jul 10;39(7):BSR20190513. doi: 10.1042/BSR20190513. PMID: 31160487; PMCID: PMC6620384.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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