Name : SARS PLpro/papain-like protease Protein
Product Source :
Recombinant SARS-CoV-2 PLpro/papain-like protease Protein is expressed from E.coil with His tag and Avi tag at the N-Terminus. It contains Glu1-Ile314.[Accession | P0C6U8]
Molecular Weight :
The protein has a predicted MW of 38.6 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Supplied as 0.22μm filtered solution in 20mM Tris, 100mM NaCl, 10% Glycerol (pH 7.8).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE SARS-CoV-2 PLpro on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Background :
The coronaviral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro), are attractive antiviral drug targets because they are essential for coronaviral replication. Although the primary function of PLpro and 3CLpro are to process the viral polyprotein in a coordinated manner, PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses.
Synonyms :
Replicase polyprotein 1a; pp1a; nsp1; PL2-PRO; PL-PRO; Papain-like Protease; Plpro
References & Citations :
(1) Báez-Santos YM, St John SE, Mesecar AD. The SARS-coronavirus papain-like protease: structure, function and inhibition by designed antiviral compounds. Antiviral Res. 2015 Mar;115:21-38. doi: 10.1016/j.antiviral.2014.12.015. Epub 2014 Dec 29. PMID: 25554382; PMCID: PMC5896749.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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