Name : Human SCF Protein
Product Source :
Recombinant Human SCF Protein is expressed from E.coli without tag. It contains Glu26-Ala189.[Accession | P21583-1]
Molecular Weight :
The protein has a predicted MW of 18.45 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human SCF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human SCF at 5μg/ml (100μl/well) on the plate. Dose response curve for Human CD117, His Tag with the EC50 of 1.6μg/ml determined by ELISA. Cell Based Assay Measured in a cell proliferation assay using Mo7e cells. The ED50 for this effect is 1-8 ng/mL (QC Test).
Background :
Kit-ligand (Kitl) also known as steel factor, stem cell factor and mast cell growth factor plays a crucial role in the development and maintenance of the melanocyte lineage in adult skin. Kitl exerts permanent survival, proliferation and migration functions in Kit receptor-expressing melanocytes.
Synonyms :
MGF; c-Kit ligand; sKITLG; KITLG; FPH2; KL-1; Kitl; SF; SHEP7; KL
References & Citations :
Wehrle-Haller B. The role of Kit-ligand in melanocyte development and epidermal homeostasis. Pigment Cell Res. 2003 Jun;16(3):287-96. doi: 10.1034/j.1600-0749.2003.00055.x. PMID: 12753403.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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