Name : Human SCF Protein

Product Source :
Recombinant Human SCF Protein is expressed from E.coli without tag. It contains Glu26-Ala189.[Accession | P21583-1]

Molecular Weight :
The protein has a predicted MW of 18.45 kDa same as Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human SCF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human SCF at 5μg/ml (100μl/well) on the plate. Dose response curve for Human CD117, His Tag with the EC50 of 1.6μg/ml determined by ELISA. Cell Based Assay Measured in a cell proliferation assay using Mo7e cells. The ED50 for this effect is 1-8 ng/mL (QC Test).

Background :
Kit-ligand (Kitl) also known as steel factor, stem cell factor and mast cell growth factor plays a crucial role in the development and maintenance of the melanocyte lineage in adult skin. Kitl exerts permanent survival, proliferation and migration functions in Kit receptor-expressing melanocytes.

Synonyms :
MGF; c-Kit ligand; sKITLG; KITLG; FPH2; KL-1; Kitl; SF; SHEP7; KL

References & Citations :
Wehrle-Haller B. The role of Kit-ligand in melanocyte development and epidermal homeostasis. Pigment Cell Res. 2003 Jun;16(3):287-96. doi: 10.1034/j.1600-0749.2003.00055.x. PMID: 12753403.

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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