Name : Mouse SEMA3A Protein
Product Source :
Recombinant Mouse SEMA3A Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Tyr21-Val772.[Accession | O08665]
Molecular Weight :
The protein has a predicted MW of 86.6 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl, 100mM L-arginine, 100mM glutamic acid (pH 8.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse SEMA3A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse SEMA3A is greater than 95% as determined by SEC-HPLC.
Background :
The CRMP proteins were originally identified as mediators of Sema3A signaling and neuronal differentiation. Much has been learned about the mechanism by which CRMPs regulate cellular responses to Sema3A.The secreted protein Sema3A (collapsin-1) was the first identified vertebrate semaphorin. Sema3A acts primarily as a repulsive axon guidance cue, and can cause a dramatic collapse of the growth cone lamellipodium.Neuropilin-1 (NP1) and members of the class A plexins (PlexA) form a Sema3A receptor complex, with NP1 serving as a high-affinity ligand binding partner, and PlexA transducing the signal into the cell via its large intracellular domain.
Synonyms :
Sema III; Semaphorin; Sema D; coll-1; collapsin 1; Hsema-I; Hsema-III; SEMA1, Sema3A; SEMAD; SEMAIII; SEMAL;
References & Citations :
(1) Schmidt EF, Strittmatter SM. The CRMP family of proteins and their role in Sema3A signaling. Adv Exp Med Biol. 2007;600:1-11. doi: 10.1007/978-0-387-70956-7_1. PMID: 17607942; PMCID: PMC2853248.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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