Name : Cynomolgus SEZ6 Protein
Product Source :
Recombinant Cynomolgus SEZ6 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Leu20-His927.[Accession | A0A2K5WPJ4]
Molecular Weight :
The protein has a predicted MW of 99.11 kDa. Due to glycosylation, the protein migrates to 150-180 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus SEZ6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus SEZ6 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus SEZ6, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-SEZ6 Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA.
Background :
Seizure-related protein 6 (Sez6) contributes to chronic pain development as sez6 knockout mice show attenuated pain behaviours after peripheral nerve injury, compared with control mice. The type I transmembrane isoform of Sez6 is cleaved by the β-amyloid precursor protein cleavage enzyme 1 (BACE1), resulting in Sez6 extracellular domain shedding from the neuron surface.
Synonyms :
SEZ-6; SEZ6; BSRPC; BSRP-C;
References & Citations :
(1) Roitman M, Edgington-Mitchell LE, Mangum J, Ziogas J, Adamides AA, Myles P, Choo-Bunnett H, Bunnett NW, Gunnersen JM. Sez6 levels are elevated in cerebrospinal fluid of patients with inflammatory pain-associated conditions. Pain Rep. 2019 Mar 25;4(2):e719. doi: 10.1097/PR9.0000000000000719. PMID: 31041421; PMCID: PMC6455686.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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