Name : Human CD209/DC-SIGN Protein
Product Source :
Recombinant Human CD209/DC-SIGN Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Gln59-Ala404.[Accession | Q9NNX6-1]
Molecular Weight :
The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CD209 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CD209 is greater than 95% as determined by SEC-HPLC. SPR Data Human BTN2A1, His Tag immobilized on CM5 Chip can bind Human CD209, His Tag with an affinity constant of 0.12 μM as determined in SPR assay (Biacore T200).
Background :
C-type lectin CD209/DC-SIGN and CD209L/L-SIGN proteins are distinct cell adhesion and pathogen recognition receptors that mediate cellular interactions and recognize a wide range of pathogens, including viruses such as SARS, SARS-CoV-2, bacteria, fungi and parasites. Pathogens exploit CD209 family proteins to promote infection and evade the immune recognition system.
Synonyms :
CD209; CLEC4L; DC-SIGN; DC-SIGN1; CDSIGN; MGC129965; CD209 molecule
References & Citations :
(1) Rahimi N. C-type Lectin CD209L/L-SIGN and CD209/DC-SIGN: Cell Adhesion Molecules Turned to Pathogen Recognition Receptors. Biology (Basel). 2020 Dec 22;10(1):1. doi: 10.3390/biology10010001. PMID: 33375175; PMCID: PMC7822156.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
LAMP3/CD208 Proteinsupplier
Cathepsin H Proteinweb
Popular categories:
Cathepsin B
Siglec-14