Name : Biotinylated Human Siglec-5/CD170 Protein (Primary Amine Labeling)
Product Source :
Recombinant Biotinylated Human Siglec-5/CD170 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Glu17-Thr434.[Accession | O15389]
Molecular Weight :
The protein has a predicted MW of 73.1 kDa. Due to glycosylation, the protein migrates to 90-115 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human Siglec-5 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Background :
Group B Streptococcus (GBS) causes invasive infections in human newborns. the GBS β-protein attenuates innate immune responses by binding to sialic acid-binding immunoglobulin-like lectin 5 (Siglec-5), an inhibitory receptor on phagocytes.the polymorphism could influence the risk of prematurity among human fetuses of mothers colonized with GBS. This first functionally proven example of a paired receptor system in the Siglec family has multiple implications for regulation of host immunity.
Synonyms :
CD170; CD33 antigen-like 2; CD33L2; OBBP2; Siglec5; SIGLEC5; OB-BP2
References & Citations :
(1) Ali SR, Fong JJ, Carlin AF, Busch TD, Linden R, Angata T, Areschoug T, Parast M, Varki N, Murray J, Nizet V, Varki A. Siglec-5 and Siglec-14 are polymorphic paired receptors that modulate neutrophil and amnion signaling responses to group B Streptococcus. J Exp Med. 2014 Jun 2;211(6):1231-42. doi: 10.1084/jem.20131853. Epub 2014 May 5. PMID: 24799499; PMCID: PMC4042635.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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