Name : Human Siglec-15/CD33L3 Protein
Product Source :
Recombinant Human Siglec-15/CD33L3 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Lys24-Gly260.[Accession | Q6ZMC9-1]
Molecular Weight :
The protein has a predicted MW of 52.6 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in 20mM NaAC (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM NaAC (pH 5.0).
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Siglec-15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human Siglec-15, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 37.3ng/ml determined by ELISA.
Background :
Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-15 consists of a 244 amino acid extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.
Synonyms :
CD33 molecule-like 3; CD33L3; HsT1361; Siglec15; CD33 antigen-like 3; SIGLEC-15
References & Citations :
(1)Jun Wang,et al.Siglec-15 as an immune suppressor and potential target for normalization cancer immunotherapy.Nature Medicine (2019).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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