Name : Human Siglec-8 Protein

Product Source :
Recombinant Human Siglec-8 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Met17-Ala363.[Accession | Q9NYZ4]

Molecular Weight :
The protein has a predicted MW of 64.6 kDa. Due to glycosylation, the protein migrates to 70-82 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human Siglec-8 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. SEC-HPLC The purity of Human Siglec-8 is greater than 95% as determined by SEC-HPLC.

Background :
Siglec-8, also known as SAF, is an approximately 75 kDa transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-8 consists of a 347 amino acid (aa) extracellular domain (ECD) with three Ig-like domains.Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid.

Synonyms :
CDw329; MGC59785; SAF2; SAF2SAF-2; Siglec-8; SIGLEC8L; SIGLEC8

References & Citations :
(1)Macauley M S , Crocker P R , Paulson J C. Siglec-mediated regulation of immune cell function in disease[J]. Nature Reviews Immunology, 2014, 14(10):653-666.

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