Name : Biotinylated Human Siglec-9 Protein
Product Source :
Recombinant Biotinylated Human Siglec-9 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gln18-Gly348.[Accession | Q9Y336-1]
Molecular Weight :
The protein has a predicted MW of 38.9 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in 25mM MES, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 25mM MES, 150 mM NaCl (pH 5.5).
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human Siglec-9 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Background :
Siglec-9 is a sialic-acid-binding lectin expressed predominantly on myeloid cells. Aberrant glycosylation occurs in essentially all types of cancers and results in increased sialylation. Thus, when the mucin MUC1 is expressed on cancer cells, it is decorated by multiple short, sialylated O-linked glycans (MUC1-ST).
Synonyms :
CD329; CDw329; FOAP-9; OBBP-Like; Siglec-9; SIGLEC9
References & Citations :
(1) Beatson R, Tajadura-Ortega V, Achkova D, Picco G, Tsourouktsoglou TD, Klausing S, Hillier M, Maher J, Noll T, Crocker PR, Taylor-Papadimitriou J, Burchell JM. The mucin MUC1 modulates the tumor immunological microenvironment through engagement of the lectin Siglec-9. Nat Immunol. 2016 Nov;17(11):1273-1281. doi: 10.1038/ni.3552. Epub 2016 Sep 5. PMID: 27595232; PMCID: PMC5257269.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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