Product Source :
Recombinant FITC-Labeled Human B7-H3 (4Ig) /B7-H3b Protein is expressed from HEK293 with hFc tag at the C-terminus. It contains Gly27-Thr461.[Accession | Q5ZPR3-1]
Molecular Weight :
The protein has a predicted MW of 73.4 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE FITC-Labeled Human B7-H3 (4Ig) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of FITC-Labeled Human B7-H3 (4Ig) is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized FITC-Labeled Human B7-H3 (4Ig), hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 62.5ng/ml determined by ELISA.
Background :
B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.
Synonyms :
B7-H3; CD276; PSEC0249; 4Ig-B7-H3; UNQ309; PRO352; B7 homolog 3; CD276
References & Citations :
(1)Fodstad O , Tekle C , Chen Y W. The Immunoregulatory Protein Human B7H3 is a Tumor-Associated Antigen that Regulates Tumor Cell Migration and Invasion[J]. Current Cancer Drug Targets, 2008, 8(5)
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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