Name : Human SMPD1 Protein
Product Source :
Recombinant Human SMPD1 Protein is expressed from Baculovirus-Insect Cells with His tag at the C-terminus. It contains Met1-Pro628.[Accession | NP_000534.3]
Molecular Weight :
The protein has a predicted MW of 66.53 kDa. Due to glycosylation, the protein migrates to 67-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Supplied as 0.22 μm filtered solution in 20mM Tris, 500mM NaCl, 25% glycerol (pH 7.5).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human SMPD1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human SMPD1 is greater than 95% as determined by SEC-HPLC. Bioactivity Data Measured by its ability to cleave 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HNPPC). The specific activity is > 1500 pmol/min/μg, as measured under the described conditions.
Background :
Sphingomyelin phosphodiesterase 1 (SMPD1) converts sphingomyelin into ceramide and phosphocholine; hence, loss of SMPD1 function causes abnormal accumulation of sphingomyelin in lysosomes, which results in the lipid-storage disorder Niemann-Pick disease (types A and B). SMPD1 activity is dependent on zinc, which is coordinated at the active site of the enzyme.
Synonyms :
ASM;
References & Citations :
Ueda S, Manabe Y, Kubo N, Morino N, Yuasa H, Shiotsu M, Tsuji T, Sugawara T, Kambe T. Early secretory pathway-resident Zn transporter proteins contribute to cellular sphingolipid metabolism through activation of sphingomyelin phosphodiesterase 1. Am J Physiol Cell Physiol. 2022 May 1;322(5):C948-C959. doi: 10.1152/ajpcell.00020.2022. Epub 2022 Mar 16. PMID: 35294847.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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