Name : Cynomolgus SOST/Sclerostin Protein
Product Source :
Recombinant Cynomolgus SOST/Sclerostin Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln69-Tyr258.[Accession | XP_005584428.2]
Molecular Weight :
The protein has a predicted MW of 22.58 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus SOST on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Cynomolgus SOST, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-SOST Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA.
Background :
SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. SOST is negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
Synonyms :
sclerostin; SOST; VBCHsclerosteosis; CDD; DAND6; SOST1; VBCH
References & Citations :
(1)Claire M N , Patton D , Hayes J S. Sclerostin Antibody Therapy for the Treatment of Osteoporosis: Clinical Prospects and Challenges[J]. Journal of Osteoporosis, 2016, 2016:1-22.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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