Name : Cynomolgus SPP1/OPN Protein
Product Source :
Recombinant Cynomolgus SPP1/OPN Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Leu17-Asn300.[Accession | A0A2K5WL90]
Molecular Weight :
The protein has a predicted MW of 33.16 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus SPP1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus SPP1 is greater than 95% as determined by SEC-HPLC.
Background :
Ovarian cancer is one of the most lethal malignant tumors in women. Secreted phosphoprotein 1 (SPP1) plays an important role in some cancer types. The expression of SPP1 was higher in epithelial ovarian cancer tissues than in normal ovarian tissues. Silencing SPP1 decreased the cell proliferation, migration, and invasion. Ectopic expression of SPP1 increased the cell proliferation, migration, and invasion. Silencing SPP1 prevented ovarian cancer growth in mice. Silencing SPP1 inhibited Integrin β1/FAK/AKT pathway.
Synonyms :
Osteopontin; Bone sialoprotein 1; Nephropontin; SPP-1; BNSP; OPN; SPP1; Uropontin; BSP-1; ETA-1; BSPI
References & Citations :
(1) Zeng B, Zhou M, Wu H, Xiong Z. SPP1 promotes ovarian cancer progression via Integrin β1/FAK/AKT signaling pathway. Onco Targets Ther. 2018 Mar 12;11:1333-1343. doi: 10.2147/OTT.S154215. PMID: 29559792; PMCID: PMC5856063.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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