Name : Biotinylated Human SIRP Beta/CD172b Protein
Product Source :
Recombinant Biotinylated Human SIRP Beta/CD172b Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu30-Ala369.[Accession | O00241-1]
Molecular Weight :
The protein has a predicted MW of 40 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human SIRP Beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human SIRP Beta is greater than 95% as determined by SEC-HPLC.
Background :
SIRP beta 1 is a type I transmembrane protein belonging to the SIRP family within the Ig superfamily. Members of this family are characterized by an extracellular region containing a V-set Ig domain containing a J-like sequence and two C1-set Ig domains.SIRP beta 1 is immunoglobulin-like cell surface receptor involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Participates also in the recruitment of tyrosine kinase SYK. Triggers activation of myeloid cells when associated with TYROBP.
Synonyms :
SIRP-beta-1 isoform 3; SIRPB1; CD172b; DKFZp686A05192; FLJ26614; 9930027N05Rik; SIRP-beta; Sirpb; Sirpb1; Sirpb1a;SIRP-β-1 isoform 3; SIRP-β
References & Citations :
(1)Takizawa H , Manz M G. Macrophage tolerance: CD47–SIRP-α–mediated signals matter[J]. Nature Immunology, 2007, 8(12):1287-1289.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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