Name : Biotinylated Human SIRP gamma/CD172g Protein
Product Source :
Recombinant Biotinylated Human SIRP gamma/CD172g Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu29-Pro360.[Accession | Q9P1W8]
Molecular Weight :
The protein has a predicted MW of 39.7 kDa. Due to glycosylation, the protein migrates to 48-53 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human SIRP gamma on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.. SEC-HPLC The purity of Biotinylated Human SIRP gamma is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Biotinylated Human SIRP Gamma, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human CD47, hFc Tag with the EC50 of 0.27μg/ml determined by ELISA.
Background :
Signal regulatory protein gamma (SIRP gamma, designated CD172g), also called SIRP beta 2, is a monomeric 45-47 kDa type I transmembrane protein belonging to the SIRP/SHPS (CD172) family of the Ig superfamily.SIRP gamma is probable immunoglobulin-like cell surface receptor. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation.
Synonyms :
CD172g; FLJ42230; SIRP beta 2; SIRP gamma; SIRP-B2; SIRPG; Cripto-1; bA77C3.1; SIRP β 2
References & Citations :
(1) Piccio, L. Adhesion of human T cells to antigen-presenting cells through SIRP beta2-CD47 interaction costimulates T-cell proliferation[J]. Blood, 2005, 105(6):2421-2427.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
Serum Albumin/ALB ProteinSpecies
ARG2 Proteinsite
Popular categories:
ErbB2/CD340
BMP-3B/GDF10