Name : Human B7-H7/HHLA2 Protein
Product Source :
Recombinant Human B7-H7/HHLA2 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ile23-Asn344.[Accession | Q9UM44-1]
Molecular Weight :
The protein has a predicted MW of 63.7 kDa. Due to glycosylation, the protein migrates to 78-100 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per ug by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human B7-H7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human B7-H7 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CD28H, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human B7-H7, hFc Tag with the EC50 of 90.0ng/ml determined by ELISA (QC Test). SPR Data Human B7-H7, hFc Tag captured on CM5 Chip via Protein A can bind Human KIR3DL3, His Tag with an affinity constant of 334 nM as determined in SPR assay (Biacore T200).
Background :
B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins.Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.
Synonyms :
B7H7; B7-H7; HHLA2; B7 Homolog 7
References & Citations :
(1)Leung J , Suh W K. The CD28-B7 Family in Anti-Tumor Immunity: Emerging Concepts in Cancer Immunotherapy[J]. Immune Network, 2014, 14(6).
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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