Name : Human TEM1/CD248 Protein

Product Source :
Recombinant Human TEM1/CD248 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln18-Leu687.[Accession | Q9HCU0-1]

Molecular Weight :
The protein has a predicted MW of 72.7 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human TEM1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human TEM1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human TEM1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TEM1 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA.

Background :
Colorectal cancer (CRC) is one of the most common cancers worldwide usually diagnosed in the advanced stage. The serum concentration of tumor endothelial marker 1 (TEM1) was measured and correlated with clinicopathological features to evaluate whether TEM1 might serve as a biomarker for early CRC diagnosis, progression, and prognosis. TEM1 can act as a potential diagnostic, progression, and prognostic serum biomarker for patients with CRC; TEM1 might be a good supplement for commonly used markers CEA and Ca 19-9.

Synonyms :
Endosialin; CD248; CD164L1; TEM1; CD164 sialomucin-like 1; CD164L1; CD248 molecule; MGC119479

References & Citations :
(1) Pietrzyk Ł, Wdowiak P. Endosialin (TEM1) as a Diagnostic, Progression, and Prognostic Serum Marker for Patients With Colorectal Cancer-A Preliminary Study. Cancer Control. 2020 Jan-Dec;27(1):1073274820903351. doi: 10.1177/1073274820903351. PMID: 32107922; PMCID: PMC7053787.

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